FastQCFastQC Report
Mon 6 Feb 2012
1.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename 1.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11268106
Filtered Sequences 0
Sequence length 50
%GC 49

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC 2239299 19.872896119365578 Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC 699683 6.20941088058632 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG 633186 5.619276211991616 TruSeq Adapter, Index 2 (100% over 49bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG 333184 2.9568766924982777 Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT 134431 1.1930221458690573 Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCACCATTTGAAATCAGTGTTAGATCGGAAGAGCACACGTCTGAACTC 100092 0.8882770538367317 Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTCCAGT 79341 0.7041201067863578 Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA 74194 0.6584425102142277 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGAACACGTCTGAACTC 69804 0.6194829903091078 Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC 52051 0.46193211174974746 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT 47738 0.4236559364989999 Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCACAGTGGCTAAGTTCCGTAGATCGGAAGAGCACACGTCTGAACTCCA 43007 0.3816701759816601 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA 40436 0.3588535642103473 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCACAGTGGCTAAGTTCCGCAGATCGGAAGAGCACACGTCTGAACTCCA 38869 0.3449470567635768 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT 34354 0.3048782111208397 Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA 33638 0.29852399329576773 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA 33630 0.2984529964485602 TruSeq Adapter, Index 2 (100% over 36bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC 33085 0.2936163362325488 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC 31687 0.28120963718303676 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG 30127 0.26736525197757277 Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT 29762 0.26412602082373027 Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC 29577 0.2624842187320567 Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGCTTATCAGACTGATGTTGAAAGATCGGAAGAGCACACGTCTGAACTC 29536 0.26212035989011817 Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC 27727 0.2460661978153205 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTCCAG 26223 0.23271879054030908 Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTAGATCGGAAGAGCACACGTCTGAACTCCAG 25980 0.23056226130638105 Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCCTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC 21461 0.19045791724004016 Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC 20457 0.18154781291549796 Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA 20027 0.17773173237809442 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC 19744 0.17522021890812883 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA 19433 0.17246021647293697 Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC 19011 0.16871513278274095 Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG 18376 0.16307975803564503 Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCNTATCAGACTGATGTTGACAGATCGGAAGAGAACACGTCTGAACTC 18278 0.16221004665735306 Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG 17688 0.15697402917579936 TruSeq Adapter, Index 2 (100% over 35bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA 16054 0.14247292313366594 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTC 15873 0.1408666194655961 Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG 15753 0.13980166675748346 TruSeq Adapter, Index 2 (100% over 38bp)
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA 14579 0.12938287942978174 Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC 14414 0.12791856945612687 TruSeq Adapter, Index 2 (100% over 50bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTAT 14013 0.12435985248985056 TruSeq Adapter, Index 2 (100% over 48bp)
TAGCCTATCAGACTGATGTTGACAGATCGGAAGAGAACACGTCTGAACTC 13550 0.12025090995771606 Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC 13362 0.11858248404833963 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTATAAAGCAATGAGACTGATTAGATCGGAAGAGCACACGTCTGAACTCC 13192 0.11707380104518009 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCC 13167 0.11685193589765662 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC 13035 0.11568048791873276 Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGATGTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG 12632 0.11210402174065455 TruSeq Adapter, Index 2 (100% over 38bp)
TAGCGTATCAGACTGATGTTGACAGATCGGAAGAGAACACGTCTGAACTC 12428 0.11029360213686311 Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TTCACAGTGGCTAAGTTCCAGATCGGAAGAGCACACGTCTGAACTCCAGT 12137 0.10771109181969 Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC 11906 0.10566105785657323 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACACAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC 11688 0.10372639377016865 Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGAGGAGCTCACAGTCTAGTAGATCGGAAGAGCACACGTCTGAACTCCA 11655 0.10343353177543768 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACTGGCCTACAAAGTCCCAGTAGATCGGAAGAGCACACGTCTGAACTCC 11497 0.10203134404308942 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC 11479 0.10187160113687252 Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGCGTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC 11295 0.10023867365109983 Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CGTCT 10743935 31.495808 423.52856 40
GTCTG 10844195 30.605124 408.52176 41
TCTGA 10962095 26.3768 347.5782 42
ACGTC 10727550 22.53844 302.71033 39
CACGT 10724120 22.531235 302.40933 38
ATCGG 10906290 22.060116 295.64877 26
GATCG 10899715 22.046814 294.8688 25
TCGGA 10870025 21.986765 287.0944 27
AGATC 10881690 18.765442 250.31256 24
CTGAA 10877650 18.758478 249.26828 43
GAACT 10770590 18.573849 248.32385 45
TGAAC 10720960 18.488262 248.66698 44
AACTC 10305110 18.458979 257.49402 46
CACAC 10473200 16.380579 208.20978 36
ACACG 10714125 16.132956 217.20207 37
AGCAC 10644230 16.027712 201.58409 34
GCACA 10495465 15.803704 201.15515 35
CGGAA 10813820 15.676311 205.67871 28
ACTCC 7148860 15.601008 147.28145 46
AGAGC 10606760 15.3761425 195.10414 32
GAGCA 10599555 15.3657 194.75905 33
GGAAG 10998450 15.349834 198.73337 29
GATGT 6336510 14.67865 438.55182 15
GAAGA 12268130 14.597625 169.14659 30
TGTTG 4429430 14.316931 606.22577 17
AAGAG 10836690 12.894382 168.93759 31
CTCCA 5647685 12.324985 89.69659 25
ATGTT 4448315 12.258274 519.3961 16
TTATC 4126195 11.810712 517.00006 5
CTTAT 3961440 11.339122 515.4937 4
CTGAT 4586910 11.036943 455.4814 13
TCCAG 5099800 10.714614 86.27344 26
TGATG 4523510 10.4788 438.1527 14
GTTGA 4472595 10.360855 432.66766 18
TAGCT 3991240 9.60365 430.62497 1
GCTTA 3946115 9.495073 432.139 3
GACTG 4660185 9.42614 383.5975 11
AGCTT 3895485 9.373247 431.31128 2
CAGTC 4454960 9.359812 79.202034 28
TCAGA 5307785 9.15326 329.53336 8
CCAGT 4288745 9.010597 79.50319 27
TATCA 4371195 8.967275 388.25574 6
ATCAG 5121085 8.831297 327.7201 7
ACTGA 4663810 8.042727 325.7936 12
TTGAC 3338010 8.031861 337.58426 19
CAGAT 4639435 8.000692 224.52594 23
AGACT 4537660 7.8251805 326.16415 10
GTCAC 3342445 7.0224323 79.18537 30
CAGAC 4509370 6.790053 284.8401 9
TCACC 3093580 6.751142 79.90041 31
AGTCA 3584365 6.1812263 65.00326 29
TGACA 3282820 5.661213 234.3905 20
CACCG 2869675 5.2644234 67.23726 32
CCGAT 2360065 4.9584656 74.22991 34
GACAG 3189880 4.624226 184.97545 21
TGTAT 1671755 4.606875 108.15455 38
CGATG 2091770 4.231016 71.22454 35
ACAGA 3348690 4.13877 157.54158 22
TAGAT 2047885 4.0445786 32.06849 22
ACCGA 2614575 3.9369361 53.129936 33
TATCT 1276675 3.654321 112.96603 40
TCTCG 1182100 3.465322 117.34322 42
ATGTA 1747705 3.4517221 74.95267 37
GTATC 1410750 3.3945217 93.786354 39
CTCGT 1092825 3.2036128 117.29074 43
AAGAT 2190205 3.1001766 60.275608 22
ATCTC 1229285 3.0723715 100.09422 41
CGTAT 1102385 2.6525397 96.66139 45
TCCGA 1197970 2.5169194 48.924747 17
TCGTA 1035795 2.492312 96.8211 44
TTGAA 1251910 2.4725258 82.37279 19
GAGAT 1489220 2.4724572 38.32099 20
GTATG 1014410 2.3499007 93.45085 46
AGTTC 962120 2.3150363 71.33013 14
ACAGT 1293295 2.2302833 52.23714 4
TGGCT 780785 2.2035773 84.35106 8
GTTCC 675840 1.9812226 87.04154 15
TTCCG 675235 1.979449 84.039406 16
TGAAG 1146025 1.9026723 66.556206 20
GTAGA 1122430 1.863499 16.752037 20
CAGTG 846295 1.711798 55.917946 5
GTGGC 713235 1.6921219 71.589264 7
GTCCG 663460 1.634957 14.311879 9
GGCTA 754780 1.5266912 60.55398 9
TTCAC 578730 1.446429 64.617165 1
AGTCC 671480 1.4107704 11.931794 8
CGATC 668235 1.4039528 11.157101 15
AAGTT 707600 1.3975121 58.207355 13
AGTGG 706655 1.376086 58.30849 6
TAAGT 681435 1.3458362 58.31227 12
TTAGA 658715 1.3009641 17.296198 21
TCACA 692315 1.240106 48.147972 2
CTAAG 703315 1.2128648 51.139988 11
TGTAG 522815 1.2111113 10.397735 6
GCTAA 685985 1.1829793 51.116596 10
TACAG 661665 1.1410394 9.776222 5
GATCA 655150 1.1298041 9.161743 16
TTTGT 288165 1.1080034 21.588379 12
TCTAC 442365 1.1056099 14.162471 3
GTTCT 326245 1.0953137 9.300927 1
TTGTA 395540 1.0899943 22.948315 13
CTACA 602070 1.0784551 10.027134 4
CCGAC 586780 1.0764488 9.889158 11
ACGAT 622750 1.0739306 9.164125 14
GACGA 739320 1.0717591 7.9929867 13
CACAG 703795 1.0597501 39.92724 3
CGAGA 724370 1.0500867 30.106993 19
TTCTA 366385 1.0487309 15.421275 2
GTAGT 445380 1.0317315 27.913576 5
GGTAG 523055 1.0185575 23.671637 4
CGACG 573890 1.0135709 9.441516 12
GTTAG 433435 1.0040607 17.502323 20
CCGAG 562655 0.99372846 36.36142 18
AGTAG 574475 0.95376426 21.577394 7
TAGTA 436910 0.86289847 25.349474 6
GTTTG 266335 0.86085564 18.242811 11
TGAGA 514795 0.8546813 8.611715 20
TGTTA 295195 0.8134724 17.065432 20
GCAGA 509260 0.7382514 5.063788 20
TCAGT 302765 0.7285078 15.213757 16
AGAGA 573955 0.68293864 11.358068 32
GTGTT 206335 0.66692185 19.95995 19
AGAAC 532675 0.65835285 11.5764885 34
TGCTG 232350 0.6557518 12.139898 16
TGAGG 333685 0.64979273 21.42555 1
TTTGA 231830 0.6388567 17.454721 9
GCTGT 223645 0.6311841 12.394026 17
GAGGT 316860 0.6170291 22.124418 2
TAGTT 223600 0.61617714 14.107734 9
GTGCT 217960 0.6151395 12.333753 15
GAACA 464960 0.5746613 11.598918 35
CTCAG 273405 0.5744204 7.9216113 11
GATTG 227410 0.52679974 12.889326 11
GCTCA 243670 0.51194745 8.19816 10
TTGAG 220760 0.5113949 11.187864 19
AGGTA 305495 0.50719386 18.689869 3
TTGTG 153575 0.49638954 14.837467 13
ATTGT 178330 0.49142608 14.9854555 12
TGAAA 344535 0.48768002 9.519856 11
AGCTC 231225 0.48580065 8.001888 9
AGTGT 209435 0.4851603 14.744736 18
CAAGA 388385 0.48001942 10.347974 23
ACAAG 388355 0.4799824 10.343967 22
GAGAA 402125 0.47848123 11.270846 33
ATTTG 164640 0.4537004 17.281485 8
CTGTA 188480 0.4535173 5.2162495 19
CTGTT 134520 0.45162868 12.490258 18
CATTT 155120 0.44401142 18.263205 7
GCACC 240960 0.44204143 11.308455 3
AACAC 339960 0.4364322 11.960858 36
AGTTT 157835 0.43494776 13.984635 10
ATAGT 218720 0.43197265 13.677076 17
TAGCA 247640 0.4270544 15.340734 1
TGTGC 149070 0.42071414 12.545108 14
TATAG 210160 0.41506663 13.627189 16
TTCAG 168985 0.40660873 7.1173835 9
AGATT 205815 0.4064852 10.093939 10
CCATT 157195 0.39287996 15.894293 6
GTATA 197070 0.3892138 13.762848 15
AAATC 260040 0.3823265 9.801074 13
GAGTT 164585 0.3812644 5.5746036 2
CAGTT 154745 0.3723447 6.9810395 6
TTGGA 159805 0.37019145 5.0424857 4
CGTAG 181635 0.3673925 5.637142 19
GTTGT 113115 0.36561355 7.4637275 12
CACCA 223945 0.35026053 9.487372 4
GAAAT 247020 0.34965017 9.337092 12
GGCTC 136605 0.3366342 6.7768035 2
AGGTT 144835 0.33551311 6.5109134 10
GGTTG 121820 0.3309961 7.5188136 11
GGGAT 168825 0.32875696 6.317256 2
GACAA 261290 0.32293808 11.0279255 21
ATAGG 189175 0.31407523 6.3455234 16
TAGGT 127510 0.29537943 6.3700223 9
TCGGG 123900 0.29394788 5.6067424 27
TCCGT 100190 0.29370663 8.786768 17
AGCCT 138590 0.29117575 8.032883 2
GGGGA 174810 0.2861585 5.392568 1
GGATG 145030 0.2824204 6.3859935 3
GTAGG 145020 0.28240088 5.707833 8
ATTCT 93170 0.26668736 7.5732703 1
ACTTT 90955 0.2603472 5.0558314 16
AGCGT 128690 0.26030082 5.669077 2
ACCAT 144255 0.2583961 10.885685 5
CCTAT 103265 0.25809187 9.478732 4
GTAGC 126455 0.2557801 7.790627 7
CTATC 102335 0.2557675 9.603127 5
GTTCA 103120 0.24812552 7.0218453 8
AATCA 168130 0.24719484 9.2292595 14
GCCTA 117095 0.24601506 8.142179 3
CTTTG 72515 0.24345711 6.1861286 17
TAGCC 115110 0.24184456 8.859019 1
CCGTA 112525 0.23641355 5.8534017 18
TCCGC 83750 0.21437246 6.5144916 17
CTTGG 75235 0.21233264 5.081113 7
AGTTA 105150 0.20767154 6.288206 19
TCAGC 96225 0.20216745 6.1194205 10
GCGTA 98205 0.19863893 5.6535573 3
TAGCG 91365 0.18480365 5.9273257 1
CTGCC 70150 0.17956093 5.16686 4
GCTGC 65735 0.16199003 5.256459 3
TTCAA 70710 0.14505781 5.3495545 1